Open AccessEndogenous pannexin1 channels form functional intercellular cell–cell channels with characteristic voltage-dependent properties
Author(s) -
Nicolás Palacios-Prado,
Paola Soto,
Ximena López,
Eun Ju Choi,
Valeria Márquez-Miranda,
Mónica Maldonado Rojas,
Yorley Duarte,
Jinu Lee,
Fernando D. González-Nilo,
Juan C. Sáez
Publication year - 2022
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2202104119
Subject(s) - gap junction , intracellular , microbiology and biotechnology , biophysics , cell culture , cell , connexin , biology , coupling (piping) , cell junction , hela , chemistry , biochemistry , materials science , genetics , metallurgy
Significance Pannexin1 is a glycoprotein that has been shown to form functional plasma membrane channels and mediate many cellular signaling pathways. However, the formation and function of pannexin1-based intercellular cell–cell channels in mammalian cells and vertebrate tissue is a question of substantial debate. This work provides robust electrophysiological evidence to demonstrate that endogenously expressed human pannexin1 forms cell–cell channels and lays the groundwork for studying a potential new type of electrical synapses between many mammalian cell types that endogenously express pannexin1.