Open AccessCSB-independent, XPC-dependent transcription-coupled repair in Drosophila
Author(s) -
Nazli Deger,
Xuemei Cao,
Christopher P. Selby,
Saygin Gulec,
Hiroaki Kawara,
Evan B. Dewey,
Li Wang,
Yanyan Yang,
Sierra J. Archibald,
Berkay Selçuk,
Ogün Adebali,
Jeff Sekelsky,
Aziz Sancar,
Zhenxing Liu
Publication year - 2022
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2123163119
Subject(s) - nucleotide excision repair , dna repair , biology , mutagenesis , genetics , drosophila (subgenus) , t cell receptor , multicellular organism , microbiology and biotechnology , dna , mutation , gene , immune system , t cell
Significance We have discovered thatDrosophila , which does not have the canonical TCR homologs, does nevertheless carry out TCR as efficiently as organisms that do. Furthermore, using the XR-seq and in vivo excision assay we have also shown that both global repair and TCR inDrosophila are dependent on the XPC protein and in that regard,Drosophila excision repair is more similar to the monocellular eukaryotic yeast repair system than it is to multicellular eukaryotes. Finally, we have generated genome-wide single nucleotide repair maps ofDrosophila for CPDs, (6-4) photoproducts, and cisplatin-d(GpG) adducts that should be a useful source for investigators working on DNA damage, repair, and mutagenesis inDrosophila .