Open AccessHigh-precision tumor resection down to few-cell level guided by NIR-IIb molecular fluorescence imaging
Author(s) -
Feifei Wang,
Liangqiong Qu,
Fuqiang Ren,
Ani Baghdasaryan,
Yingying Jiang,
RuSiou Hsu,
Liang Peng,
Jiachen Li,
Guanzhou Zhu,
Zhuoran Ma,
Hongjie Dai
Publication year - 2022
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2123111119
Subject(s) - autofluorescence , fluorescence lifetime imaging microscopy , molecular imaging , fluorescence , preclinical imaging , in vivo , materials science , luminescence , chemistry , biomedical engineering , optics , medicine , optoelectronics , biology , physics , microbiology and biotechnology
Significance Surgical removal of tumors has been performed to combat cancer for over a century by surgeons relying on visual inspection and experience to identify margins between malignant and healthy tissues. Herein, we present a rare-earth down-conversion nanoparticle–anti-CD105 conjugate for cancer targeting and a handheld imager capable of concurrent photographic imaging and fluorescence/luminescence imaging. An unprecedented tumor-to-muscle ratio was achieved by near-infrared-IIb (NIR-IIb, 1,500 to 1,700 nm) imaging during surgery, ∼100 times higher than previous organic dyes for unambiguous determination of tumor margin. The sensitivity/biocompatibility/safety of the probes and instrumentation developed here open a paradigm of imaging-guided surgery at the single-cell level, meeting all major requirements for clinical translation to combat cancer and save human lives.