RibU is an essential determinant of Listeria pathogenesis that mediates acquisition of FMN and FAD during intracellular growth | Zendy

Rafael RiveraLugo | Zendy; S.H. Light | Zendy; Nicholas E. Garelis | Zendy; Daniel A. Portnoy | Zendy

Open Access

RibU is an essential determinant of Listeria pathogenesis that mediates acquisition of FMN and FAD during intracellular growth

Author(s) -

Rafael RiveraLugo,

S.H. Light,

Nicholas E. Garelis,

Daniel A. Portnoy

Publication year - 2022

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.2122173119

Subject(s) - riboflavin , flavin group , auxotrophy , flavin mononucleotide , flavin adenine dinucleotide , cofactor , biology , biochemistry , mutant , microbiology and biotechnology , intracellular , listeria monocytogenes , b vitamins , intracellular parasite , flavoprotein , bacteria , gene , enzyme , genetics , endocrinology

Significance Riboflavin (vitamin B2 ) is converted into flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which are essential cofactors for many redox reactions across all domains of life.Listeria monocytogenes is a facultative intracellular pathogen that cannot synthesize riboflavin and must therefore obtain flavins from the host. In this study, we show that a previously identified riboflavin transporter (RibU) is essential for virulence and intracellular growth, but rather than transporting riboflavin, RibU transports FMN and FAD directly from the host cell cytosol. Mutants unable to convert riboflavin to FMN and FAD retained their capacity to grow intracellularly and were virulent, but they were unable to grow extracellularly and were thus converted from facultative to obligate intracellular pathogens.

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