Open AccessLipid membranes modulate the activity of RNA through sequence-dependent interactions
Author(s) -
Tomasz Czerniak,
James Sáenz
Publication year - 2022
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2119235119
Subject(s) - riboswitch , ribozyme , rna , ligase ribozyme , biology , non coding rna , biochemistry , chemistry , microbiology and biotechnology , biophysics , gene
Significance Lipids, which are essential for life and one of the most ancient biomolecules, can spontaneously self-assemble to form membranous bilayers, theoretically providing a surface that can serve to concentrate, protect, and regulate RNAs. Here, we show that direct RNA–lipid interactions can modulate ribozyme activity in a lipid-dependent manner. We further demonstrate that RNA–lipid binding is influenced by nucleotide content and base pairing of RNAs. This yields an essential framework for engineering RNA–lipid systems that can be regulated based on sequence specificity and introduces a mechanism for riboregulation in cellular, synthetic, and prebiotic systems.