Open AccessEukaryotic catecholamine hormones influence the chemotactic control of Vibrio campbellii by binding to the coupling protein CheW
Author(s) -
Angela Weigert Muñoz,
Elisabeth Hoyer,
Kilian Schumacher,
Marianne Grognot,
Katja M. Taute,
Stephan M. Hacker,
Stephan A. Sieber,
Kirsten Jung
Publication year - 2022
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2118227119
Subject(s) - chemotaxis , biology , hormone , vibrio , microbiology and biotechnology , agonist , receptor , bacteria , biochemistry , genetics
Significance Host-emitted stress hormones significantly influence the growth and behavior of various bacterial species; however, their cellular targets have so far remained elusive. Here, we used customized probes and quantitative proteomics to identify the target of epinephrine and the α-adrenoceptor agonist phenylephrine in live cells of the aquatic pathogenVibrio campbellii . Consequently, we have discovered the coupling protein CheW, which is in the center of the chemotaxis signaling network, as a target of both molecules. We not only demonstrate direct ligand binding to CheW but also elucidate how this affects chemotactic control. These findings are pivotal for further research on hormone-specific effects on bacterial behavior.