Open AccessDeciphering the endometrial niche of human thin endometrium at single-cell resolution
Author(s) -
Haining Lv,
Guangfeng Zhao,
Pengju Jiang,
Huiyan Wang,
Zhiyin Wang,
Simin Yao,
Zhenhua Zhou,
Limin Wang,
Dan Liu,
Wenbo Deng,
Jianwu Dai,
Yali Hu
Publication year - 2022
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2115912119
Subject(s) - endometrium , stromal cell , biology , decidualization , cell growth , cell , microbiology and biotechnology , andrology , endocrinology , cancer research , medicine , genetics
Significance Thin endometrium is the most common reason for uterine infertility and refractory gynecological diseases due to its complexity in pathogenesis and adverse pregnancy outcomes. Here, we profile cells from normal and thin endometrium at single-cell resolution to investigate the sophisticated alterations in the local microenvironment that occur in thin endometrium. Increased cellular senescence, collagen overdeposition, and significant down-regulation of gene expression related to cell proliferation are observed and confirmed. Moreover, we demonstrate aberrant activation of the SEMA3 pathway accompanied by dampened EGF, PTN, and TWEAK signaling pathways in thin endometrium. These findings aid in understanding the mechanisms of thin endometrium and provide new tools to rejuvenate the atrophic endometrium for female fertility preservation and successful pregnancy.