Viable offspring derived from single unfertilized mammalian oocytes | Zendy

Yanchang Wei | Zendy; CaiRong Yang | Zendy; ZhenAo Zhao | Zendy

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Viable offspring derived from single unfertilized mammalian oocytes

Author(s) -

Yanchang Wei,

CaiRong Yang,

ZhenAo Zhao

Publication year - 2022

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.2115248119

Subject(s) - biology , oocyte , genomic imprinting , parthenogenesis , epigenetics , somatic cell nuclear transfer , methylation , dna methylation , offspring , genetics , imprinting (psychology) , zygote , embryo , microbiology and biotechnology , andrology , embryogenesis , dna , blastocyst , gene , gene expression , pregnancy , medicine

Significance In mammals, parthenogenesis is limited because of problems arising from genomic imprinting. Here, we report live mammalian offspring derived from single unfertilized eggs. This was achieved by the targeted DNA methylation rewriting of seven imprinting control regions. By designing guide RNAs with protospacer adjacent motif (PAM) sequences matching one allele but not the other, dCas9-Dnmt3a or dCpf1-Tet1 enables targeted DNA methylation editing in an allele-specific manner. The success of parthenogenesis in mammals opens many opportunities in agriculture, research, and medicine.

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