Open AccessViable offspring derived from single unfertilized mammalian oocytes
Author(s) -
Yuanchen Wei,
Chingwen Yang,
Zhen-Ao Zhao
Publication year - 2022
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2115248119
Subject(s) - biology , oocyte , genomic imprinting , parthenogenesis , epigenetics , somatic cell nuclear transfer , methylation , dna methylation , offspring , genetics , imprinting (psychology) , zygote , embryo , microbiology and biotechnology , andrology , embryogenesis , dna , blastocyst , gene , gene expression , pregnancy , medicine
Significance In mammals, parthenogenesis is limited because of problems arising from genomic imprinting. Here, we report live mammalian offspring derived from single unfertilized eggs. This was achieved by the targeted DNA methylation rewriting of seven imprinting control regions. By designing guide RNAs with protospacer adjacent motif (PAM) sequences matching one allele but not the other, dCas9-Dnmt3a or dCpf1-Tet1 enables targeted DNA methylation editing in an allele-specific manner. The success of parthenogenesis in mammals opens many opportunities in agriculture, research, and medicine.