Open AccessEngineering and functional analysis of yeast with a monotypic 40S ribosome subunit
Author(s) -
Xin Hu,
Shuangying Jiang,
Feifei Xu,
Cheng Zeng,
Xiangxiang Wang,
Wei Liu,
Aimin Cheng,
Chunyang Ma,
Ning Gao,
Yuyu Zhao,
Junbiao Dai,
Guanghou Zhao
Publication year - 2022
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2114445119
Subject(s) - biology , eukaryotic small ribosomal subunit , ribosome , genetics , gene , ribosomal protein , phenotype , subfunctionalization , ribosomal rna , computational biology , microbiology and biotechnology , gene expression , gene family , rna
Significance Ribosomes are not monolithic but dynamic machines composed of heterogeneous ribosomal protein (RP) paralogs with elusive functions. Isolation and characterization of monotypic ribosomes with homogeneous RP paralog compositions represent ideal approaches to understand the role of pervasive RP paralogs in customizing translation abilities but are largely hurdled by the complexity of the cellular ribosome pool (e.g., inSaccharomyces cerevisiae , 59 RP paralog pairs allow >1017 potential RP combinations). Here, we engineered a yeast with monotypic 40S ribosomes, including both defined and homogenous RP paralogs, and further functional studies revealed that duplicated RP paralogs impart robustness and phenotypic plasticity (such as paromomycin tolerance) through both gene dose amplification and paralog-specific regulation, paving a way for the study of monotypic ribosomes.