Open AccessCircadian key component CLOCK/BMAL1 interferes with segmentation clock in mouse embryonic organoids
Author(s) -
Yasuhiro Umemura,
Nobuya Koike,
Yoshiki Tsuchiya,
Hidenobu Watanabe,
Gen Kondoh,
Ryoichiro Kageyama,
Kazuhiro Yagita
Publication year - 2021
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2114083119
Subject(s) - circadian clock , biology , somitogenesis , oscillating gene , circadian rhythm , clock , microbiology and biotechnology , mesoderm , bacterial circadian rhythms , neuroscience , embryonic stem cell , genetics , embryo , embryogenesis , somite , gene
Significance Although the circadian clock is essential for regulating the temporal order of physiological functions, circadian oscillation is strictly suppressed in the early-to-mid–stage embryos in mammalian developmental process. The biological significance controlling the suppression of the circadian clock and its delayed emergence in mammalian embryos has been unknown. Here, we show that the premature expression of the functional circadian components CLOCK/BMAL1 in mouse induced presomitic mesoderm and gastruloids can interfere with the segmentation clockHes7 oscillation and somitogenesis through theHes7 transcription and its regulatory pathways. This suggests that the CLOCK/BMAL1 function may need to be suppressed during somitogenesis.