Open AccessHost cytoskeletal vimentin serves as a structural organizer and an RNA-binding protein regulator to facilitate Zika viral replication
Author(s) -
Yue Zhang,
Shuangshuang Zhao,
Yian Li,
Fengping Feng,
Min Li,
Yanhong Xue,
Jie Cui,
Tao Xu,
Xia Jin,
Yaming Jiu
Publication year - 2022
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2113909119
Subject(s) - vimentin , biology , viral replication , microbiology and biotechnology , cytoskeleton , intermediate filament , rna , viral structural protein , virology , viral entry , virus , genetics , cell , gene , immunohistochemistry , immunology
Significance We discovered a dual role of vimentin underlying Zika virus (ZIKV) replication. The vimentin network reorganizes to surround the replication complex. Depletion of vimentin resulted in drastic segregation of viral proteins and subsequent defective infection, indicating its function as an “organizer” that ensures the concentration of all necessary factors for high replication efficacy. With omics analysis, we prove that vimentin also functions as a “regulator” that dominates RNA-binding proteins during infection. These two roles complement one another to make an integrated view of vimentin in regulating ZIKV infection. Collectively, our study fills the long-term gap in our knowledge of the cellular function of intermediate filaments in addition to structural support and provides a potential target for ZIKV therapy.