Open AccessPhenotypic determinism and stochasticity in antibody repertoires of clonally expanded plasma cells
Author(s) -
Daniel Neumeier,
Alexander Yermanos,
Andreas Agrafiotis,
Lucia Csepregi,
Tasnia Chowdhury,
Roy Ehling,
Raphael Kuhn,
Tudor-Stefan Cotet,
Raphaël B. Di Roberto,
Mariangela Di Tacchio,
Renan Antonialli,
Dale Starkie,
Daniel Lightwood,
Annette Oxenius,
Sai T. Reddy
Publication year - 2022
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2113766119
Subject(s) - somatic hypermutation , biology , epitope , antibody repertoire , antigen , affinity maturation , antibody , polyclonal antibodies , repertoire , immunodominance , genetics , virology , b cell , physics , acoustics
Significance B cell clonal selection and expansion from a genetically diverse antibody repertoire guides the immune response to a target antigen. It remains unclear if clonal selection and expansion follow any deterministic rules or are stochastic with regards to phenotypic antibody properties such as antigen-binding, affinity, and epitope specificity. We perform the in-depth genotypic and phenotypic characterization of antibody repertoires following immunization in mice. We identify the degree to which clonal expansion is driven by antibody binding, affinity, and epitope specificity and as such may provide greater insight into vaccine-induced immunity.