Open AccessKARRIKIN UP-REGULATED F-BOX 1 (KUF1) imposes negative feedback regulation of karrikin and KAI2 ligand metabolism in Arabidopsis thaliana
Author(s) -
Claudia Sepúlveda,
Michael Guzmán,
Qingtian Li,
José Antonio Villaécija-Aguilar,
Stephanie E. Martinez,
Muhammad Kamran,
Aashima Khosla,
Wei Liu,
Joshua M. Gendron,
Caroline Gutjahr,
Mark T. Waters,
David C. Nelson
Publication year - 2022
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2112820119
Subject(s) - arabidopsis thaliana , strigolactone , ligand (biochemistry) , biology , microbiology and biotechnology , metabolism , signal transduction , arabidopsis , negative feedback , biochemistry , gene , receptor , mutant , physics , quantum mechanics , voltage
Significance Karrikins are chemicals in smoke that stimulate regrowth of many plants after fire. However, karrikin responses are not limited to species from fire-prone environments and can affect growth after germination. Putatively, this is because karrikins mimic an unknown signal in plants, KAI2 ligand (KL). Karrikins likely require modification in plants to become bioactive. We identify a gene,KUF1 , that appears to negatively regulate biosynthesis of KL and metabolism of a specific karrikin.KUF1 expression increases in response to karrikin or KL signaling, thus forming a negative feedback loop that limits further activation of the signaling pathway. This discovery will advance understanding of how karrikins are perceived and how smoke-activated germination evolved. It will also aid identification of the elusive KL.