Open AccessStructural insights into how vacuolar sorting receptors recognize the sorting determinants of seed storage proteins
Author(s) -
Hsi-En Tsao,
Shu Nga Lui,
Anthony Hiu-Fung Lo,
Shuai Chen,
Hiu Yan Wong,
Chun Kit Wong,
Liwen Jiang,
KamBo Wong
Publication year - 2021
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2111281119
Subject(s) - arabidopsis , vacuole , biochemistry , storage protein , protein targeting , biology , c terminus , gene isoform , microbiology and biotechnology , chemistry , amino acid , cytoplasm , gene , membrane protein , mutant , membrane
Significance Seeds such as rice and soybean are major food staples in the human diet. During seed development, storage proteins are deposited in a specialized organelle called the protein storage vacuole and are mobilized to provide nutrients during germination. Storage proteins are transported as cargoes via specific protein–protein interactions with the vacuolar sorting receptors. Supported by structural and mutagenesis studies, our work provides insights into how the sequence-specific information, or the vacuolar sorting determinant, on the storage proteins is recognized by the vacuolar sorting receptors for their targeting to the vacuoles. Insights gained into the rules of receptor–cargo recognition will be useful in engineering recombinant proteins for biotechnological applications of the protein storage vacuoles in seeds.