Open AccessAnnealing synchronizes the 70 S ribosome into a minimum-energy conformation
Author(s) -
Xiaofeng Chu,
Xin Su,
Mingdong Liu,
Li Li,
Tianhao Li,
Yicheng Qin,
Guoliang Lü,
Lei Qi,
Yunhui Liu,
Jinzhong Lin,
Qing-Tao Shen
Publication year - 2022
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2111231119
Subject(s) - ribosome , crystallography , chemical physics , annealing (glass) , energy landscape , cryo electron microscopy , materials science , biophysics , chemistry , physics , biological system , biology , thermodynamics , rna , biochemistry , gene
Significance In metallurgy, annealing heats a metal or alloy to a predetermined temperature, holds this temperature for a certain time, and then decreases the metal to room temperature to change the physical and sometimes also the chemical properties of the material. Researchers introduce a similar concept to simulated annealing to predict minimum-energy conformations of biological macromolecules. In this work, we experimentally verify that annealing at a fast cooling rate can synchronize the 70S ribosome into a nonrotated state with minimum energy in our free-energy landscape analysis. Our results not only offer a facile yet robust approach to stabilizing proteins for high-resolution structural analysis but also contribute to understanding of protein folding and temperature adaptation.