Open AccessKinetic proofreading of lipochitooligosaccharides determines signal activation of symbiotic plant receptors
Author(s) -
Kira Gysel,
Mette Laursen,
Mikkel B. Thygesen,
Damiano Lironi,
Zoltán Bozsóki,
Christian T. Hjuler,
Nicolai N. Maola,
Jeryl Cheng,
Peter Björk,
Maria Vinther,
Lene Heegaard Madsen,
Henriette Rübsam,
Artur Muszyński,
Arshia Ghodrati,
Parastoo Azadi,
John T. Sullivan,
Clive W. Ronson,
Knud J. Jensen,
Mickaël Blaise,
Simona Radutoiu,
Jens Stougaard,
Kasper R. Andersen
Publication year - 2021
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2111031118
Subject(s) - receptor , biophysics , biology , signal transduction , microbiology and biotechnology , biochemistry , in silico , pattern recognition receptor , transmembrane protein , receptor–ligand kinetics , computational biology , gene , innate immune system
Significance Plant cell surface receptors perceive carbohydrate signaling molecules and hereby establish communication with surrounding microbes. Genetic studies have identified two different classes of lysin motif receptor kinases as gatekeepers that together trigger the symbiotic pathway in plants; however, no structural or functional data of the perception mechanisms switching these receptors from resting state into activation is known. In this study, we use structural biology, biochemical, and genetic approaches to demonstrate how the NFP/NFR5 class of lipochitooligosaccharide (LCO) receptors discriminate bacterial symbionts based on a kinetic proofreading mechanism that controls receptor activation and signaling specificity. We show that the LCO binding site can be engineered to support symbiotic functions, which greatly advance future opportunities for receptor engineering in legumes and nonlegumes.