Open AccessCircadian pacemaker neurons display cophasic rhythms in basal calcium level and in fast calcium fluctuations
Author(s) -
Xitong Liang,
Timothy E. Holy,
Paul H. Taghert
Publication year - 2022
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2109969119
Subject(s) - calcium , t type calcium channel , circadian rhythm , voltage dependent calcium channel , biology , p type calcium channel , r type calcium channel , ryanodine receptor , calcium channel , calcium in biology , calcium signaling , endocrinology , medicine , neuroscience
Significance Daily rhythms in the molecular clock, in calcium, and in electrical activity all interact to support the functions of circadian pacemaker neurons. However, the regulatory mechanisms that unify these properties are not defined. Here, we utilize the cellular resolution of theDrosophila circadian neural circuit with technological improvements in light-sheet imaging. We report that individualDrosophila pacemakers display two cophasic rhythms of daily calcium fluctuations. We previously described the first: slow changes in intracellular calcium. The second involves high-frequency calcium fluctuations that depend on the function of the T-type calcium channel. We propose that the fast rhythms, emerging sequentially across the 24-h day, correspond to spontaneous electrical activity patterns displayed by different pacemaker groups.