Open AccessCD169+macrophages in lymph node and spleen critically depend on dual RANK and LTbetaR signaling
Author(s) -
Abdouramane Camara,
Alice C. Lavanant,
Jun Abe,
Henri Lee Desforges,
Yannick O. Alexandre,
Erika Girardi,
Zinaida Igamberdieva,
Kenichi Asano,
Masato Tanaka,
Thomas Hehlgans,
Klaus Pfeffer,
Sébastien Pfeffer,
Scott N. Mueller,
Jens V Stein,
Christopher G. Mueller
Publication year - 2022
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2108540119
Subject(s) - spleen , microbiology and biotechnology , biology , rankl , lymph node , marginal zone , stromal cell , macrophage , immune system , immunology , cellular differentiation , cd11c , receptor , cancer research , b cell , antibody , biochemistry , in vitro , phenotype , activator (genetics) , gene
Significance The CD169+ macrophages that play an important role in the fight against infections and cancer are receptive to environmental signals for their differentiation. We show that lymph node and splenic CD169+ macrophages require both LTβR and RANK signaling since the conditional deficiency of either receptor results in their disappearance. Using a reporter mouse, we observe RANKL expression by a splenic mesenchymal cell subset and show that it participates in CD169+ macrophage differentiation. Their absence leads to a reduced viral capture and a greatly attenuated virus-specific CD8+ T cell expansion. Thus, tight control mechanisms operate for the precise positioning of these macrophages at sites where numerous immune-stimulatory forces converge.