Open AccessSequential in vivo labeling of insulin secretory granule pools inINS-SNAPtransgenic pigs
Author(s) -
Elisabeth Kemter,
Andreas Müller,
Martin Neukam,
Anna Ivanova,
Nikolai Klymiuk,
Simone Renner,
Kaiyuan Yang,
Johannes Broichhagen,
Mayuko Kurome,
Valeri Zakhartchenko,
Barbara Keßler,
Klaus-Peter Knoch,
Marc Bickle,
Barbara Ludwig,
Kai Johnsson,
Heiko Lickert,
Thomas Kurth,
Eckhard Wolf,
Michele Solimena
Publication year - 2021
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2107665118
Subject(s) - insulin , granule (geology) , transgene , in vivo , genetically modified mouse , biology , diabetes mellitus , microbiology and biotechnology , secretion , stimulation , medicine , endocrinology , biochemistry , genetics , gene , paleontology
Significance The failure of β cells to secrete sufficient amounts of insulin is a key feature of diabetes mellitus. Each β cell secretes only a small amount of insulin upon stimulation in a highly regulated fashion: young insulin is preferentially released, whereas old insulin is mainly degraded within the β cell. How this process is regulated in vivo and likely altered in diabetes is currently unknown. We present here a transgenic pig model that allows the in vivo fluorescent labeling of age-distinct insulin secretory granule pools, hence providing a close-to-life readout of insulin turnover. This will enable the study of alterations in β cell function in an animal model close to humans.