English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Significance Primer-dependent transcription initiation—the use of RNA primers as initiating entities in transcription initiation—yields RNA products having a 5′-hydroxyl. Here, we show that primer-dependent initiation in vivo inEscherichia coli involves predominantly dinucleotide primers, involves any of the 16 possible dinucleotide primers, and depends on promoter sequences in, upstream, and downstream of the primer binding site. Crystal structures explain the structural basis of sequence dependence at the promoter position immediately upstream of the primer binding site, namely, interchain base stacking between the promoter template-strand nucleotide and primer 5′ nucleotide. Taken together, our findings provide a mechanistic and structural description of primer-dependent initiation inE. coli .

Promoter-sequence determinants and structural basis of primer-dependent transcription initiation in Escherichia coli