Host’s guardian protein counters degenerative symbiont evolution

Significance When laying eggs, plataspid stinkbugs deposit small packets called “symbiont capsules.” Newborn stinkbugs suck the capsules to acquire a bacterial mutualist. Without the symbiont, the babies cannot grow and die. Due to the long-lasting symbiosis, the symbiont has experienced genome reduction and become uncultivable. Within the capsules, however, the symbiont can survive for over a week outside the host. Why and how? Here, we uncover a molecular secret implemented in the symbiont capsules. Mother stinkbugs massively produce a special intestinal secretion protein, PMDP, at the expense of their own survival. PMDP embeds the fragile symbiont cells and protects them within the capsules. The host-provisioned molecule for sustaining symbiosis may be utilized for cultivation and/or preservation of fastidious microorganisms.