Open AccessThymine dissociation and dimer formation: A Raman and synchronous fluorescence spectroscopic study
Author(s) -
Anushka Nagpal,
Dinesh Dhankhar,
Thomas C. Cesario,
Runze Li,
Jie Chen,
P. M. Rentzepis
Publication year - 2021
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2025263118
Subject(s) - thymine , photochemistry , pyrimidine dimer , dimer , dissociation (chemistry) , chemistry , fluorescence , raman spectroscopy , dna , nucleobase , dna repair , biochemistry , organic chemistry , physics , quantum mechanics , optics
Significance Bacteria inactivation by ultraviolet light is caused due to the formation of thymine dimers that inhibit DNA replication. We have used spectroscopic means for the identification of thymine dimer(s) that may pave the way for fast and definite identification of the ratio of live and dead bacteria, as suggested by our previous spectroscopic studies. In the present study, the dissociation of thymine to thymine dimers and (6-4) photoproducts was detected by means of a Raman, absorption, and synchronous fluorescence study. These data provide a fast method for determining, in situ, the reaction mechanism and final photoproducts formed as a function of UV irradiation dose.