Open AccessEndothelial cell invasion is controlled by dactylopodia
Author(s) -
A. Figueiredo,
Pedro Barbacena,
Ana Russo,
Silvia Vaccaro,
Daniela Ramalho,
Andreia Pena,
Aida P. Lima,
Rita R. Ferreira,
Marta F Fidalgo,
Fatima El-Marjou,
Yulia Carvalho,
Francisca F. Vasconcelos,
Ana-María Len-Duménil,
Danijela Matic Vignjevic,
Cláudio A. Franco
Publication year - 2021
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2023829118
Subject(s) - filopodia , microbiology and biotechnology , angiogenesis , sprouting angiogenesis , extracellular matrix , endothelial stem cell , cell migration , rac1 , biology , neovascularization , actin , cell , signal transduction , cancer research , biochemistry , genetics , in vitro
Significance In this report, we describe how endothelial cells, the cells lining the interior of blood vessels, invade into tissues to form new vessels through sprouting angiogenesis. We found that endothelial cells use a specific lamellipodia-related membrane protrusion for invasion, which we termed dactylopodia. These protrusions have a special morphology, originate from filopodia, are linked to membrane-ruffling activity, and are specialized in invading into avascular extracellular matrix. Our work lays the foundations for drug discovery targeting sprouting angiogenesis.