Open AccessAcetogenic bacteria utilize light-driven electrons as an energy source for autotrophic growth
Author(s) -
Sangrak Jin,
Yale Jeon,
Min Soo Jeon,
Jongoh Shin,
Yoseb Song,
Sung-Jin Kang,
Jiyun Bae,
Suhyung Cho,
Jung-Kul Lee,
Dong Rip Kim,
Byung-Kwan Cho
Publication year - 2021
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2020552118
Subject(s) - nanoparticle , electron transfer , artificial photosynthesis , photosynthesis , biophysics , nanotechnology , chemistry , cadmium sulfide , flavin group , cathode , electron , chemical engineering , materials science , photochemistry , biochemistry , biology , inorganic chemistry , physics , photocatalysis , engineering , enzyme , catalysis , quantum mechanics
Significance To develop an efficient artificial photosynthesis system using acetogen-nanoparticle hybrids, the efficiency of the electron–hole pair generation of nanoparticles must be enhanced to demonstrate extracellular electron utilization by the acetogen. Here we verified thatClostridium autoethanogenum , an industrially relevant acetogen, could use electrons generated from size- and structure-controlled chemically synthesized cadmium sulfide nanoparticles displayed on the cell surface under light-exposure conditions. In addition, transcriptomic analysis showed that the electrons generated from nanoparticles were largely transported to the intracellular matrix via the metal ion or flavin-binding proteins. These results illustrate the potential to increase the CO2 -fixing efficiency of nanoparticle-based artificial photosynthesis by engineering cellular processes related to electron transfer generated from the cathode.