Open AccessReal-time measurements of aminoglycoside effects on protein synthesis in live cells
Author(s) -
Javier Aguirre Rivera,
Jimmy Larsson,
Ivan L. Volkov,
A. Carolin Seefeldt,
Suparna Sanyal,
Magnus Johansson
Publication year - 2021
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2013315118
Subject(s) - aminoglycoside , protein biosynthesis , antibiotics , bacterial protein , chemistry , kinetics , microbiology and biotechnology , biophysics , biology , biochemistry , gene , physics , quantum mechanics
Significance To address the public health challenges caused by the spread of antibiotic resistance, it is critical to enhance our understanding of the mechanisms of action of these compounds. In the present study, we use superresolved single-molecule tracking techniques, to investigate the effect of three aminoglycoside drugs on protein synthesis kinetics directly inside live bacterial cells. Our results imply that these drugs do not completely inhibit bacterial protein synthesis, but only make it slower. Hence, the bactericidal effect of these drugs is likely due to a disturbed, rather than inhibited, protein synthesis process.