The SKI complex is a broad-spectrum, host-directed antiviral drug target for coronaviruses, influenza, and filoviruses | Zendy

Stuart Weston | Zendy; Lauren Baracco | Zendy; Chloe Keller | Zendy; Krystal Matthews | Zendy; Marisa E. McGrath | Zendy; James Logue | Zendy; Janie Liang | Zendy; Julie Dyall | Zendy; Michael R. Holbrook | Zendy; Lisa E. Hensley | Zendy; Peter B. Jahrling | Zendy; Wenbo Yu | Zendy; Alexander D. MacKerell | Zendy; Matthew B. Frieman | Zendy

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The SKI complex is a broad-spectrum, host-directed antiviral drug target for coronaviruses, influenza, and filoviruses

Author(s) -

Stuart Weston,

Lauren Baracco,

Chloe Keller,

Krystal Matthews,

Marisa E. McGrath,

James Logue,

Janie Liang,

Julie Dyall,

Michael R. Holbrook,

Lisa E. Hensley,

Peter B. Jahrling,

Wenbo Yu,

Alexander D. MacKerell,

Matthew B. Frieman

Publication year - 2020

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.2012939117

Subject(s) - virology , pandemic , antiviral drug , broad spectrum , host factor , covid-19 , coronavirus , viral replication , ebola virus , influenza a virus , biology , host factors , host (biology) , antiviral therapy , virus , computational biology , medicine , infectious disease (medical specialty) , genetics , chemistry , disease , pathology , combinatorial chemistry , chronic hepatitis

The SARS-CoV-2 pandemic has made it clear that we have a desperate need for antivirals. We present work that the mammalian SKI complex is a broad-spectrum, host-directed, antiviral drug target. Yeast suppressor screening was utilized to find a functional genetic interaction between proteins from influenza A virus (IAV) and Middle East respiratory syndrome coronavirus (MERS-CoV) with eukaryotic proteins that may be potential host factors involved in replication. This screening identified the SKI complex as a potential host factor for both viruses. In mammalian systems siRNA-mediated knockdown of SKI genes inhibited replication of IAV and MERS-CoV. In silico modeling and database screening identified a binding pocket on the SKI complex and compounds predicted to bind. Experimental assays of those compounds identified three chemical structures that were antiviral against IAV and MERS-CoV along with the filoviruses Ebola and Marburg and two further coronaviruses, SARS-CoV and SARS-CoV-2. The mechanism of antiviral activity is through inhibition of viral RNA production. This work defines the mammalian SKI complex as a broad-spectrum antiviral drug target and identifies lead compounds for further development.

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