Open AccessTrichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences
Author(s) -
Wan-Chen Li,
Chia-Yi Lee,
Wei-Hsuan Lan,
Tai-Ting Woo,
Hou-Cheng Liu,
HsinYi Yeh,
Hao-Yen Chang,
Yu-Chien Chuang,
Chiung-Ya Chen,
Chi-Ning Chuang,
Chia-Ling Chen,
YiPing Hsueh,
HungWen Li,
Peter Chi,
Ting-Fang Wang
Publication year - 2021
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2007192118
Subject(s) - rad51 , biology , homologous recombination , meiosis , trichoderma reesei , saccharomyces cerevisiae , genetics , recombinase , flp frt recombination , genetic recombination , microbiology and biotechnology , dna , yeast , recombination , gene , cellulose , biochemistry , cellulase
Significance Sexual eukaryotes fall into two groups with respect to their RecA-like recombinases. The first group possesses Rad51 (ubiquitous) and Dmc1 (meiosis-specific), which cooperate to conduct interhomolog recombination in zygotes with high sequence heterogeneity. Interestingly, Dmc1 was lost from the second group of eukaryotic organisms. Here we used the industrial workhorse fungusTrichoderma reesei to address if and how Rad51-only eukaryotes carry out hybrid meiosis. We show thatT. reesei Rad51 (Tr Rad51) is indispensable for interhomolog recombination during meiosis and thatTr Rad51, likeSaccharomyces cerevisiae Dmc1, possesses a better mismatch tolerability thanS. cerevisiae Rad51. Our results indicate that the ancestralTr Rad51 evolved to acquire Dmc1-like properties by adopting multiple structural variations in the L1 and L2 DNA-binding loops.