Open AccessChannelrhodopsin-mediated optogenetics highlights a central role of depolarization-dependent plant proton pumps
Author(s) -
Antonella Reyer,
Melanie Häßler,
Sönke Scherzer,
Shouguang Huang,
Jesper Torbøl Pedersen,
Khaled A. S. Al-Rascheid,
Ernst Bamberg,
Michael G. Palmgren,
Ingo Drèyer,
Georg Nagel,
Rainer Hedrich,
Dirk Becker
Publication year - 2020
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.2005626117
Subject(s) - optogenetics , depolarization , channelrhodopsin , membrane potential , biophysics , plasmodesma , hyperpolarization (physics) , biology , electrophysiology , neuroscience , microbiology and biotechnology , chemistry , organic chemistry , cytoplasm , nuclear magnetic resonance spectroscopy
Significance People for centuries are puzzled how living creatures like plants sense their environment. Plants employ electrical signals to communicate a cue-dependent local status between plants cells and organs. As a first response to biotic and abiotic stresses, the membrane potential of plant cells depolarizes. Recovery from the depolarized state, repolarization, was proposed to involve ion channels and pumps. Here, we established channelrhodopsin (ChR2)-based optogenetics in plants and learned that the plant plasma membrane H+ -ATPase represents the major driver of membrane potential repolarization control during plant electrical signaling, rather than voltage-dependent ion channels.