Induction of recurrent break cluster genes in neural progenitor cells differentiated from embryonic stem cells in culture | Zendy

Aseda Tena | Zendy; Yuxiang Zhang | Zendy; Nia Kyritsis | Zendy; Anne Devorak | Zendy; Jeffrey Zurita | Zendy; PeiChi Wei | Zendy; Frederick W. Alt | Zendy

Open Access

Induction of recurrent break cluster genes in neural progenitor cells differentiated from embryonic stem cells in culture

Author(s) -

Aseda Tena,

Yuxiang Zhang,

Nia Kyritsis,

Anne Devorak,

Jeffrey Zurita,

PeiChi Wei,

Frederick W. Alt

Publication year - 2020

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.1922299117

Subject(s) - biology , progenitor cell , embryonic stem cell , neural stem cell , stem cell , gene , progenitor , microbiology and biotechnology , genetics

Significance We previously discovered a set of long neural genes susceptible to frequent DNA breaks in primary mouse brain progenitor cells. We termed these genes RDC-genes. RDC-gene breakage during brain development might alter neural gene function and contribute to neurological diseases and brain cancer. To provide an approach to characterize the unknown mechanism of neural RDC-gene breakage, we asked whether RDC-genes appear in neural progenitors differentiated from embryonic stem cells in culture. Indeed, robust RDC-genes appeared in neural progenitors differentiated in culture and many overlapped with robust RDC-genes in primary brain progenitors. These studies indicate that in vitro development of neural progenitors provides a model system for elucidating how RDC-genes are formed.

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