Open AccessInvestigation of phase transitions of lipids and lipid mixtures by sensitivity differential scanning calorimetry.
Author(s) -
Susan Mabrey,
Julian M. Sturtevant
Publication year - 1976
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.73.11.3862
Subject(s) - dipalmitoylphosphatidylcholine , differential scanning calorimetry , thermotropic crystal , miscibility , phase diagram , calorimetry , phase (matter) , chemistry , phase transition , analytical chemistry (journal) , chromatography , materials science , thermodynamics , phospholipid , organic chemistry , phosphatidylcholine , membrane , liquid crystalline , biochemistry , physics , polymer
High sensitivity differential scanning calorimetry is applied to the study of the thermotropic behavior of mixtures of synthetic phospholipids in multilamellar aqueous suspensions. The systems dimyristoylphosphatidylcholine dipalmitoylphosphatidylcholine, and dimyristoylphosphatidylethanolamine-distearoylphosphatidylcholine, although definitely nonideal, exhibit essentially complete miscibility in both gel and liquid crystalline states, while the system dilauroylphosphatidylcholine-distearoylphosphatidylcholine is monotectic with lateral phase separation in the gel state. Comparison of the observed transition curves with theoretical curves calculated from the calorimetrically determined phase diagrams supports a literal interpretation of the phase diagrams.