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Genetic Diversity of Picea asperata Populations Based on RAPDs
Author(s) -
Xue X.,
Wang Y.,
Korpelainen H.,
Li C.
Publication year - 2007
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1055/s-2006-924455
Subject(s) - biology , genetic diversity , rapd , gene flow , analysis of molecular variance , loss of heterozygosity , genetic variability , genetic variation , population , genetic distance , genetics , evolutionary biology , gene , allele , genotype , demography , sociology
The genetic diversity of ten natural populations of Picea asperata Mast. were studied using RAPD markers. A total of 160 reproducible fragments were produced from the ten primers used. The mean number of fragments detected per individual was 114.7. Altogether 120 fragments were polymorphic among the ten populations, none of them were found to be population‐specific. Nei's expected heterozygosity (H e ) ranged from 0.233 to 0.269, and the average was 0.247. The analysis of molecular variance revealed that the coefficient of gene differentiation among populations, based on F ST and the unbiased estimate Φ st , equaled 0.224 and 0.290, respectively. Such high values indicate that there is significant differentiation among populations, which could result from several factors, including restricted gene flow between populations ( N m = 0.866). Founder events may be another factor attributing to the high level of genetic differentiation. In addition, it was discovered that the geographic distribution is not correlated with the genetic distances among the populations of P. asperata .