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Correlated Expression of gfp and Bt cry1Ac Gene Facilitates Quantification of Transgenic Hybridization between Brassica s
Author(s) -
Shen B.C.,
Stewart C. N.,
Zhang M.Q.,
Le Y.T.,
Tang Z.X.,
Mi X.C.,
Wei W.,
Ma K.P.
Publication year - 2006
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1055/s-2006-924277
Subject(s) - cry1ac , biology , green fluorescent protein , transgene , brassica , hybrid , genetically modified crops , gene , gene flow , bacillus thuringiensis , microbiology and biotechnology , fluorescence in situ hybridization , gene expression , botany , genetics , genetic variation , bacteria , chromosome
Gene flow from transgenic oilseed rape (Brassica napus) might not be avoidable, thus, it is important to detect and quantify hybridization events with its relatives in real time. Data are presented showing the correlation between genetically linked green fluorescent protein (GFP) with Bacillus thuringiensis (Bt) cry1Ac gene expression in hybrids formed between transgenic B. napus “Westar” and a wild Chinese accession of wild mustard (B. juncea) and hybridization between transgenic B. napus and a conspecific Chinese landrace oilseed rape. Hybrids were obtained either by spontaneous hybridization in the field or by hand‐crossing in a greenhouse. In all cases, transgenic hybrids were selected by GFP fluorescence among seedlings originating from seeds harvested from B. juncea and the Chinese oilseed rape plants. Transgenicity was confirmed by PCR detection of transgenes. GFP fluorescence was easily and rapidly detected in the hybrids under greenhouse and field conditions. Results showed that both GFP fluorescence and Bt protein synthesis decreased as either plant or leaf aged, and GFP fluorescence intensity was closely correlated with Bt protein concentration during the entire vegetative lifetime in hybrids. These findings allow the use of GFP fluorescence as an accurate tool to detect gene‐flow in time in the field and to conveniently estimate Bt cry1Ac expression in hybrids on‐the‐plant.