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Construction of a Primary RH Panel of Italian Ryegrass Genome via UV‐Induced Protoplast Fusion
Author(s) -
Cheng A.,
Cui H.,
Xia G.
Publication year - 2006
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1055/s-2006-924276
Subject(s) - protoplast , biology , somatic fusion , introgression , hybrid , lolium multiflorum , somatic cell , electrofusion , common wheat , genome , chloroplast dna , lolium , botany , genetics , poaceae , gene , chromosome , materials science , metallurgy
Symmetric and asymmetric somatic hybrids were produced via protoplast fusion between common wheat ( Triticum aestivum L.) cv. “Jinan 177” and Italian ryegrass ( Lolium multiflorum Lam.). The ryegrass without or with UV irradiation was used as a donor, providing a small amount of chromatin. In these somatic hybrids, most ryegrass chromosomes have been confirmed preferential elimination and the somatic hybrid calli and plants showed wheat‐like morphology. Some of the hybrid lines were used for the analysis of distribution and heredity of donor DNA in the hybrid genome and the possibility of establishing a radiation hybrid (RH) panel of the ryegrass in the present experiment. These hybrids, subcultured for two and three years, retained the ryegrass DNA examined by RFLP and GISH analysis, respectively. Distribution of the ryegrass DNA in the wheat genomes of 20 single‐cell individuals, randomly selected from hybrid cell lines produced, were analyzed by 21 ryegrass genome specific SSR markers. The average frequencies of molecular marker retention in symmetric hybrid lines (UV 0), as well as asymmetric hybrid lines from UV 30 s and 1 min were 10.88, 15.48 and 33.86, respectively. It was suggested that the UV dose increased the introgression of donor DNA into wheat genome. The ryegrass SSR fragments in most asymmetric hybrid cell lines remained stable over a period of 2 – 3 years. This revealed that those asymmetric somatic hybrids are suitable for the introgression of ryegrass DNA into wheat, and for RH panel and RH mapping.