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Abscisic Acid Stimulates the Expression of Two Isoforms of Plasma Membrane Ca 2+ ‐ATPase in Arabidopsis thaliana Seedlings
Author(s) -
Cerana M.,
Bonza M. C.,
Harris R.,
Sanders D.,
Michelis M. I.
Publication year - 2006
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1055/s-2006-924111
Subject(s) - abscisic acid , arabidopsis thaliana , biology , gene isoform , atpase , gene expression , arabidopsis , biochemistry , microbiology and biotechnology , botany , gene , enzyme , mutant
At‐ ACA8 and At‐ ACA9 are two plasma membrane (PM) Ca 2+ ‐ATPases of Arabidopsis thaliana . In this article the expression of At‐ ACA8, At‐ ACA9, and of At‐ ACA10, a third isoform of Ca 2+ ‐ATPase closely related to PM Ca 2+ ‐ATPases, was analysed and the effect of the hormone abscisic acid (ABA) on the expression level of PM Ca 2+ ‐ATPase specific transcripts was investigated. In adult plants of A. thaliana , At‐ ACA8 and At‐ ACA10 are expressed in all organs considered whereas At‐ ACA9 is expressed only in flowers. All isoforms of PM Ca 2+ ‐ATPases can be detected in young seedlings but the amount of At‐ ACA9 mRNA is much lower than those of At‐ ACA8 and At‐ ACA10. ABA markedly and rapidly stimulates the expression of both At‐ ACA8 and At‐ ACA9 genes in young seedlings but not that of At‐ ACA10. ABA also increases the level of At‐ ACA8 protein at the PM, suggesting a role for PM Ca 2+ ‐ATPases in ABA signalling.