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Actin Organization During Eucalyptus Root Hair Development and Its Response to Fungal Hypaphorine
Author(s) -
Dauphin A.,
De Ruijter N. C. A.,
Emons A. M. C.,
Legué V.
Publication year - 2006
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1055/s-2006-923767
Subject(s) - biology , root hair , tip growth , actin , eucalyptus globulus , cytoskeleton , microbiology and biotechnology , botany , actin cytoskeleton , pisolithus , elongation , eucalyptus , pollen tube , biochemistry , symbiosis , mycorrhiza , cell , genetics , gene , pollen , materials science , pollination , ultimate tensile strength , bacteria , metallurgy
The fungus Pisolithus microcarpus establishes an ectomycorrhiza with Eucalyptus globulus . This symbiosis involves a fungal synthesis and secretion of hypaphorine, an indolic compound. Previous studies have shown that hypaphorine induces an alteration in the actin cytoskeleton of elongating root hairs and inhibits hair elongation. Using an alternative approved method, we analyzed the effects of hypaphorine on the E. globulus root hair cyto‐architecture and actin configuration in more detail and provide new results. One mM hypaphorine stops root hair elongation within 20 min, and changes the hair cyto‐architecture. Semi‐quantitative analysis of the actin cytoskeleton before and after treatment with hypaphorine shows that hypaphorine induces a shift from fine F‐actin to F‐actin bundles in the sub‐apex of the hair, which occurs first in the mid‐plane of the cell. This creates a sub‐apical cell centre free of filamentous actin, an actin configuration that differs from that during developmental growth arrest. The mechanism of action of hypaphorine is discussed.