Premium
Vectors for RNAi Technology in Poplar
Author(s) -
Meyer S.,
Nowak K.,
Sharma V. K.,
Schulze J.,
Mendel R. R.,
Hänsch R.
Publication year - 2004
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1055/s-2004-815729
Subject(s) - rna interference , biology , gene silencing , reporter gene , transformation (genetics) , gene , agrobacterium , rna silencing , microbiology and biotechnology , genetics , gene expression , computational biology , rna
The potential of double‐stranded RNA interference (RNAi) technology was studied for down‐regulation of gene expression in poplar. A set of vectors was constructed generating RNAs capable of duplex formation of sequences specific for the β‐glucuronidase (GUS) reporter gene system. These gene cassettes are driven by the CaMV‐35S promoter. To address the question of gene silencing, we tested the functionality of these vectors, both in transient assays by transforming protoplasts with the RNAi constructs, and in stably transformed GUSexpressing poplar plants. Agrobacterium ‐mediated transformation of those GUS‐expressing plants with a GUS‐specific RNAi construct showed a strong down‐regulation of the reporter gene. From these results we conclude that RNAi is also functional in poplar.