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Mtha 1, a Plasma Membrane H + ‐ATPase Gene from Medicago truncatula, Shows Arbuscule‐Specific Induced Expression in Mycorrhizal Tissue
Author(s) -
Krajinski F.,
Hause B.,
GianinazziPearson V.,
Franken P.
Publication year - 2002
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1055/s-2002-37407
Subject(s) - medicago truncatula , biology , gene , gene expression , rna , complementary dna , medicago , atpase , microbiology and biotechnology , messenger rna , mycorrhiza , botany , biochemistry , genetics , symbiosis , enzyme , bacteria
Transport processes between plant and fungal cells are key elements in arbuscular mycorrhiza (AM), where H + ‐ATPases are considered to be involved in active uptake of nutrients from the symbiotic interface. Genes encoding H + ‐ATPases were identified in the genome of Medicago truncatula and three cDNA fragments of the H + ‐ATPase gene family ( Mtha 1 ‐ 3) were obtained by RT‐PCR using RNA from M. truncatula mycorrhizal roots as template. While Mtha 2 and Mtha 3 appeared to be constitutively expressed in roots and unaffected by AM development, transcripts of Mtha 1 could only be detected in AM tissues and not in controls. Further analyses by RT‐PCR revealed that Mtha 1 transcripts are not detectable in shoots and phosphate availability did not affect RNA accumulation of the gene. Localization of transcripts by in situ hybridization on AM tissues showed that Mtha 1 RNA accumulates only in cells containing fungal arbuscules. This is the first report of arbuscule‐specific induced expression of a plant H + ‐ATPase gene in mycorrhizal tissues.