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Linear Relation between Cell Growth and Water Channels Conducted Lp in Nitellopsis
Author(s) -
Zhu G. L.,
Zhu M. J.,
Ye Q.,
Li S.,
Zhu R.,
Cao Y.,
Chen J.,
Wang X. C.
Publication year - 2002
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1055/s-2002-34131
Subject(s) - turgor pressure , plant stem , biology , biophysics , confocal laser scanning microscopy , cell wall , cell growth , confocal , botany , biochemistry , optics , physics
The effect of Lp on growth of internodes of Nitellopsis obtusa (Desv. In Lois) J. Gr. was studied, with treatments of HgCl 2 and antibodies against aquaporins (anti‐RD28) on the plasmalemma, using laser scanning confocal microscopy, and simultaneous monitoring of cell growth, turgor, E m and Lp using a turgor/membrane potential probe (T‐EP probe). Laser scanning confocal immunofluorescent microscopy indicated the immunoreaction of anti‐RD28 on the plasma membrane. Experiments showed that, following treatment with 25 μM HgCl 2 , cell growth was entirely and Lp largely inhibited, and no growth recovery was seen even when Lp was completely reversed by replacement of HgCl 2 with β‐mercaptoethanol (ME). Anti‐RD28 inhibited both Lp and growth of internodes of N. obtusa by about 80 %, and the possible influences on cell growth of changes in turgor, respiration and pH in cell walls, being excluded during treatment. It was confirmed experimentally that enlargement growth of internodes of N. obtusa was linearly related to cell Lp.