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Phenylalanine Ammonia‐Lyase Activity in Soybean Roots Extract Measured by Reverse‐Phase High Performance Liquid Chromatography
Author(s) -
Ferrarese M. L. L.,
Rodrigues J. D.,
FerrareseFilho O.
Publication year - 2000
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1055/s-2000-9162
Subject(s) - glycine , high performance liquid chromatography , phenylalanine ammonia lyase , chromatography , phenylalanine , elution , biology , methanol , ammonia , column chromatography , ferulic acid , biochemistry , chemistry , amino acid , organic chemistry
The high performance liquid chromatography (HPLC) technique was applied to measure phenylalanine ammonia‐lyase (PAL, EC 4.3.1.5) activity in soybean ( Glycine max L. Merril cv. BR16) roots. t ‐Cinnamate, the catalytic product of the PAL reaction was quantified at 275nm by isocratic elution with methanol: water through an ODS(M) column. Comparative experiments were carried out with 1.0 mM ferulic acid, an inducer of PAL activity. The results suggest that liquid chromatography is a rapid and sensitive method to analyze PAL activity in non‐purified extract.