Phenylalanine Ammonia‐Lyase Activity in Soybean Roots Extract Measured by Reverse‐Phase High Performance Liquid Chromatography | Zendy

Ferrarese M. L. L. | Zendy; Rodrigues J. D. | Zendy; FerrareseFilho O. | Zendy

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Phenylalanine Ammonia‐Lyase Activity in Soybean Roots Extract Measured by Reverse‐Phase High Performance Liquid Chromatography

Author(s) -

Ferrarese M. L. L.,

Rodrigues J. D.,

FerrareseFilho O.

Publication year - 2000

Publication title -

plant biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.871

H-Index - 87

eISSN - 1438-8677

pISSN - 1435-8603

DOI - 10.1055/s-2000-9162

Subject(s) - glycine , high performance liquid chromatography , phenylalanine ammonia lyase , chromatography , phenylalanine , elution , biology , methanol , ammonia , column chromatography , ferulic acid , biochemistry , chemistry , amino acid , organic chemistry

The high performance liquid chromatography (HPLC) technique was applied to measure phenylalanine ammonia‐lyase (PAL, EC 4.3.1.5) activity in soybean ( Glycine max L. Merril cv. BR16) roots. t ‐Cinnamate, the catalytic product of the PAL reaction was quantified at 275nm by isocratic elution with methanol: water through an ODS(M) column. Comparative experiments were carried out with 1.0 mM ferulic acid, an inducer of PAL activity. The results suggest that liquid chromatography is a rapid and sensitive method to analyze PAL activity in non‐purified extract.

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