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Phosphate Induces Rapid H 2 O 2 Generation in Soybean Suspension Cells
Author(s) -
Shigaki T.,
Bhattacharyya M. K.
Publication year - 2000
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1055/s-2000-9160
Subject(s) - pseudomonas syringae , phosphate , biology , nadph oxidase , reactive oxygen species , respiratory burst , glycine , oxidase test , pathogen , strain (injury) , biochemistry , microbiology and biotechnology , enzyme , amino acid , anatomy
Involvement of reactive oxygen species has been implicated in plant defence against pathogens. We report here a novel pathway of H 2 O 2 generation induced by the addition of phosphate in soybean ( Glycine max L.) cell suspension cultures. This H 2 O 2 generation was initiated shortly after the addition of phosphate, and lasted only approximately one hour, as opposed to several hours observed during an attack by an avirulent strain of the bacterial pathogen Pseudomonas syringae pv. glycinea (Psg). In addition, when cell cultures were treated with both phosphate and the avirulent pathogen, two distinct oxidative burst events were observed. In contrast to DPI‐sensitive Psg ‐induced H 2 O 2 generation, phosphate‐induced H 2 O 2 generation was insensitive to this NADPH oxidase inhibitor. This suggests that an NADPH oxidase‐independent pathway may be involved in the phosphate‐induced H 2 O 2 accumulation, which could be involved in sensing of phosphate availability in the environment.