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Phenolic and Oxidative Metabolism as Bioindicators of Nitrogen Deficiency in French Bean Plants ( Phaseolus vulgaris L. cv. Strike)
Author(s) -
Sánchez E.,
Soto J. M.,
García P. C.,
LópezLefebre L. R.,
Rivero R. M.,
Ruiz J. M.,
Romero L.
Publication year - 2000
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1055/s-2000-3699
Subject(s) - phaseolus , abiotic component , point of delivery , biology , abiotic stress , nitrogen , metabolism , bioindicator , botany , oxidative stress , nutrient , nitrogen cycle , horticulture , food science , biochemistry , chemistry , ecology , gene , organic chemistry
The aim of the present work was to determine the effect of abiotic stress, such as nitrogen (N) deficiency, on phenol and oxidative metabolism. In addition, we analyzed whether the response of the two metabolic processes is a good bioindicator of N deficiency in French bean plants. The N was applied to the nutrient solution in the form of NH 4 NO 3 at 1.35 mM (N1), 2.7 mM (N2) and 5.4 mM (N3), this latter dosage being considered optimal. The results indicated that application of 1.35 and 2.70 mM of N can be defined as suboptimal or deficient, as it depressed foliar biomass of the French bean plants in our experiment. In addition, abiotic stress from the application of these N dosages stimulated the enzymes PPO, POD and CAT, and inhibited PAL and SOD activities, resulting in the lowest foliar accumulation of phenolic compounds and H 2 O 2 .