Open AccessIsolation and Antimicrobial Activity of β-Sitosterol-3-O-Glucoside from Lannea Kerstingii Engl. & K. Krause (Anacardiacea)
Author(s) -
Ngaitad S. Njinga,
MI Sule,
U. U. Pateh,
H. N. A. Hassan,
Sa'ad T Abdullahi,
R N Ache
Publication year - 2016
Publication title -
journal of health and allied sciences nu
Language(s) - English
Resource type - Journals
eISSN - 2582-4953
pISSN - 2582-4287
DOI - 10.1055/s-0040-1708607
Subject(s) - antimicrobial , minimum inhibitory concentration , minimum bactericidal concentration , proteus mirabilis , chemistry , agar diffusion test , candida albicans , agar dilution , microbiology and biotechnology , phytochemical , traditional medicine , agar , staphylococcus aureus , chromatography , biology , bacteria , biochemistry , medicine , genetics
The emergence of more and more drug resistance bacteria has led to the study of the antimicrobial activity of the compound isolated from Lannea kerstingii Engl. & K. Krause (Anacardiacea) since the active principles of many drugs found in plants are secondary metabolites. A compound was isolated using dry vacuum liquid chromatography and eluting with CHCl3 -EtOAc and monitored using TLC. 3 1 13 The glycoside was characterized using 1 H NMR and 13 C NMR spectra recorded in DMSO-d6 at 400 MHz and 125 MHz, respectively. The antimicrobial activity of the compound was determined using agar diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal/minimum fungicidal concentration (MBC/MFC) was determined using broth dilution method. The compound isolated was found to be β-sitosterol-3-O-glucoside. The β-sitosterol-3-O-glucoside (200μg/ml) was active against S. aureus, Methicillin Resistant Staphylococcus aureus, P. mirabilis, S. typhi, K. pneumoniae, E. coli, B. subtilis with zone of inhibition ranging from 24mm to 34mm and inactive against P. aeroginosa and Proteus vulgaris. It was also active against the fungi C. albicans and C. tropicalis but inactive against C. krusei. The MIC ranged from 25 to 50 μg/ml while the MBC/MFC ranged from 50 to 200 μg/ml. These results show the wide spectrum antimicrobial activity of β-sitosterol-3-O-glucoside.