Open AccessPlacental Passage of Protopine in an Ex Vivo Human Perfusion System
Author(s) -
Deborah Spiess,
Vanessa Fabienne Abegg,
Antoine Chaveau,
Andrea Treyer,
Mouhssin Oufir,
Michael Reinehr,
Elisa Duong,
Olivier Potterat,
Matthias Hamburger,
Ana Paula Simões-Wüst
Publication year - 2022
Publication title -
planta medica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.422
H-Index - 116
eISSN - 1439-0221
pISSN - 0032-0943
DOI - 10.1055/a-1829-9546
Subject(s) - ex vivo , protopine , perfusion , fetus , in vivo , endocrinology , parenchyma , pharmacology , chemistry , biology , medicine , pregnancy , alkaloid , pathology , stereochemistry , genetics , microbiology and biotechnology
The placental passage of protopine was investigated with a human ex vivo placental perfusion model. The model was first validated with diazepam and citalopram, two compounds known to cross the placental barrier, and antipyrine as a positive control. All compounds were quantified by partially validated U(H)PLC-MS/MS bioanalytical methods. Protopine was transferred from the maternal to the fetal circuit, with a steady-state reached after 90 min. This study compound did not affect placental viability or functionality, as glucose consumption, lactate production, and beta-human chorionic gonadotropin, and leptin release remained constant. Histopathological evaluation of all placental specimens showed unremarkable, age-appropriate parenchymal maturation with no pathologic findings.