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In Vitro Evaluation of the Effect of Dimethyl Sulfoxide on Equine Articular Cartilage Matrix Metabolism
Author(s) -
Smith Christine L.,
Macdonald Melinda H.,
Tesch Anthony M.,
Willits Neil H.
Publication year - 2000
Publication title -
veterinary surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 79
eISSN - 1532-950X
pISSN - 0161-3499
DOI - 10.1053/jvet.2000.5607
Subject(s) - cartilage , chondrocyte , metabolism , dimethyl sulfoxide , medicine , andrology , explant culture , biochemistry , in vitro , chemistry , anatomy , organic chemistry
Objective— To evaluate the effects of dimethyl sulfoxide (DMSO) on equine articular cartilage matrix metabolism. Study Design— Using a cartilage explant culture system, proteoglycan (PG) synthesis, PG release, lactate metabolism, chondrocyte viability, and metabolism recovery were determined after cartilage exposure to DMSO. Sample Population Cartilage harvested from metacarpophalangeal and metatarsophalangeal joints of 12 horses (age range, 1 to 10 years). Methods— Explants were exposed to concentrations of DMSO (1% to 20%) for variable times (3 to 72 hours). PG synthesis and release were determined by a radiolabel incorporation assay and dimethylmethylene blue (DMMB) dye assay, respectively. Lactate released into culture media was measured, and chondrocyte viability was assessed using the Formizan Conversion Assay and a paravital staining protocol. Metabolism recovery was assessed in explants that were allowed to recover in maintenance media after exposure to DMSO. Results— PG synthesis and lactate metabolism were inhibited in a dose‐ and time‐dependent manner after exposure to DMSO concentrations > 5%; there was no significant alteration in PG release. No change in chondrocyte viability was detected after incubation with DMSO. PG synthesis and lactate metabolism returned to baseline rates when allowed a recovery period after exposure to DMSO. Conclusions— DMSO concentrations > 5% suppress equine articular cartilage matrix metabolism. Suppression of PG synthesis and lactate metabolism is reversible and does not appear to be the result of chondrocyte death. Clinical Relevance— Equine clinicians adding DMSO to intraarticular lavage solutions should be aware that DMSO may have deleterious effects on equine articular cartilage matrix metabolism.