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Modulated bone matrix‐related gene expression is associated with differences in interfacial strength of different implant surface roughness
Author(s) -
Ogawa Takahiro,
Sukotjo Cortino,
Nishimura Ichiro
Publication year - 2002
Publication title -
journal of prosthodontics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.902
H-Index - 60
eISSN - 1532-849X
pISSN - 1059-941X
DOI - 10.1053/jopr.2002.129772
Subject(s) - implant , extracellular matrix , osteocalcin , osseointegration , dentistry , gene expression , matrix (chemical analysis) , biomedical engineering , materials science , chemistry , medicine , microbiology and biotechnology , biology , gene , alkaline phosphatase , surgery , composite material , biochemistry , enzyme
Purpose The objective of this study was to examine the effect of implant surface topography on biomechanical strength and expression patterns of bone extracellular matrix (ECM)‐related genes during implant healing. Materials and Methods Cylinder implants with turned or dual acid‐etched (DE) surface were placed into the femurs of female Sprague‐Dawley rats. At 2 and 4 weeks after the implantation, the implant push‐in test was performed to measure the load‐bearing strength of the bone‐tissue interface. T‐shaped hollow implants were separately placed and the total RNA was extracted from the ingrown tissue within the hollow chamber. The RNA was subjected to reverse transcriptase polymerase chain reaction to analyze the expression pattern of selected bone extracellular matrix‐related genes. Results The push‐in value of the DE implant is approximately 300% greater than that of the turned implant at weeks 2 and 4 (p=.0090). The expression of collagen II, osteocalcin and biglycan was higher in the week 2 DE implant group than the turned implant group. At week 4 the expression of collagen II and IX was 7‐ and 26‐fold, respectively, greater in the DE implant group than in the turned implant group. Conclusions The surface topography of implants may induce the phenotypic alteration of wound healing cells. The increased interfacial strength of the DE implant may be associated with the modulated expression of the selected set of bone extracellular matrix genes.