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Presence of methylated arginine derivatives in orthotopic human liver transplantation: Relevance for liver function
Author(s) -
MartínSanz Paloma,
Olmedilla Luis,
Dulin Elena,
Casado Marta,
Callejas Nuria A.,
PérezPeña José,
Garutti Ignacio,
Sanz Javier,
Calleja Javier,
Barrigón Santos,
Boscá Lisardo
Publication year - 2003
Publication title -
liver transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.814
H-Index - 150
eISSN - 1527-6473
pISSN - 1527-6465
DOI - 10.1053/jlts.2003.50008
Subject(s) - asymmetric dimethylarginine , liver transplantation , medicine , arginine , catabolism , nitric oxide , transplantation , liver function , orthotopic liver transplantation , biochemistry , pharmacology , endocrinology , metabolism , chemistry , amino acid
Orthotopic liver transplantation (OLT) is a frequent option in the treatment of liver diseases. During the cold ischemia period of the donor liver, there is an accumulation of metabolites that are potent inhibitors of the cytokine‐inducible and endothelial nitric oxide synthase isoenzymes. We identified the presence of L ‐ N ‐monomethylarginine and asymmetric dimethylarginine (ADMA) as the main inhibitors by means of analytic high‐pressure liquid chromatography and mass spectrometry techniques. An average ADMA concentration of 450 μmol/L was measured in the preservation medium of donor livers with poor outcomes after OLT. A statistically significant relationship was observed between the concentration of methylated arginine derivatives in the graft and liver function after OLT. These data suggest that measurement of methylated arginine, released after liver protein catabolism, might provide an indication of functional status of the liver that can help the development of strategies intended to improve graft viability.