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Derivation, characterization, and phenotypic variation of hepatic progenitor cell lines isolated from adult rats
Author(s) -
Yin Li,
Sun Mingzeng,
Ilic Zoran,
Leffert Hyam L.,
Sell Stewart
Publication year - 2002
Publication title -
hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.488
H-Index - 361
eISSN - 1527-3350
pISSN - 0270-9139
DOI - 10.1053/jhep.2002.31355
Subject(s) - progenitor cell , biology , matrigel , hepatocyte , progenitor , hepatic stellate cell , microbiology and biotechnology , phenotype , population , cell culture , cell , haematopoiesis , stem cell , immunology , gene , in vitro , genetics , endocrinology , medicine , environmental health
Liver progenitor cells (LPCs) cloned from adult rat livers following allyl alcohol injury express hematopoietic stem cell and early hepatic lineage markers when cultured on feeder layers; under these conditions, neither mature hepatocyte nor bile duct, Ito, stellate, Kupffer cell, or macrophage markers are detected. These phenotypes have remained stable without aneuploidy or morphological transformation after more than 100 population doublings. When cultured without feeder layers, the early lineage markers disappear, and mature hepatocyte markers are expressed; mature hepatocytic differentiation and cell size are also augmented by polypeptide and steroidal growth factors. In contrast to hepatocytic potential, duct‐like structures and biliary epithelial markers are expressed on Matrigel. Because they were derived without carcinogens or mutagens, these bipotential LPC lines provide novel tools for models of cellular plasticity and hepatocarcinogenesis, as well as lines for use in cellular transplantation, gene therapy, and bioreactor construction.