Open AccessBiosynthesis of reduced graphene oxide using Turbinaria ornata and its cytotoxic effect on MCF‐7 cells
Author(s) -
Smita K. M.,
Abraham L. Stanley,
Kumar V. Ganesh,
Vasantharaja Raguraman,
Thirugnanasambandam R.,
Antony Ajit,
Govindaraju K.,
Velan T. Senthil
Publication year - 2021
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/nbt2.12057
Subject(s) - graphene , zeta potential , fourier transform infrared spectroscopy , biocompatibility , nuclear chemistry , mtt assay , nanomaterials , chemistry , viability assay , oxide , cytotoxicity , mcf 7 , materials science , nanotechnology , chemical engineering , organic chemistry , biochemistry , in vitro , nanoparticle , cancer cell , biology , human breast , engineering , cancer , genetics
Graphene‐based nanomaterials are gaining importance in biomedicine because of their large surface areas, solubility, and biocompatibility. Green synthesis is the most economical method for application, as it is rapid and sustainable. Biofunctionalized reduced graphene oxide (TrGO) nanosheets were synthesized using methanol extract of Turbinaria ornata , and bioreduction of graphene oxide was primarily confirmed and characterized using UV‐visible, Fourier transform infrared (FTIR), and X‐ray diffraction spectroscopy and further characterized by zeta potential and transmission electron microscopy. The FTIR spectra of TrGO showed a decrease in the band intensities of oxygen groups, thus confirming effective deoxygenation. The zeta potential value of −34.6 mV revealed that synthesized TrGO was highly stable. The cytotoxic effect of TrGO against MCF‐10A and MCF‐7 cells was ascertained using MTT assay, showed a greater cytotoxic effect on MCF‐7 cells. The IC 50 of TrGO treatment against MCF‐7 was calculated to be 31.25 µg, which is onefold lower than the cytotoxic effect of methanolic extract of T. ornata (60.0 ± 1.14 µg/ml). In addition, there was a statistically significant difference in cell viability between MCF‐10A and MCF‐7 cells in the treatment of TrGO. Hence, this study results in an efficient green reductant for producing rGO nanosheets that possess cytotoxicity against breast cancer cells.