Open AccessDevelopment of a sustainable route for the production of high‐fructose syrup from the polyfructan inulin
Author(s) -
Saikia Kongkona,
Radhakrishnan Hridya,
Rathankumar Abiram Karanam,
Senthil Kumar Siva Gokul,
Kalita Shravani,
George Jenet,
Subramanian Sivanesan,
Kumar Vaidyanathan Vinoth
Publication year - 2021
Publication title -
iet nanobiotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.366
H-Index - 38
eISSN - 1751-875X
pISSN - 1751-8741
DOI - 10.1049/nbt2.12031
Subject(s) - inulinase , inulin , fructose , glutaraldehyde , hydrolysis , chemistry , yield (engineering) , chromatography , food science , biochemistry , materials science , metallurgy
The authors used mesoporous silica microspheres as a support for the immobilization of inulinase from Aspergillus brasiliensis MTCC 1344 by the process of cross‐linking. Under optimized operating conditions of pH 6.0, particle/enzyme ratio of 2.0:1.0 and glutaraldehyde concentration of 7 mM, a maximum immobilization yield of 90.7% was obtained after a cross‐linking time of 12.25 h. Subsequently, the cross‐linked inulinase was utilized for the hydrolysis of 5% inulin, and a maximum fructose concentration of 31.7 g/L was achieved under the optimum conditions of pH 6.0 and temperature 60°C in 3 h. Furthermore, on performing reusability studies during inulin hydrolysis, it was observed that the immobilized inulinase could be reused up to 10 subsequent cycles of hydrolysis, thus providing a facile and commercially attractive process of high‐fructose syrup production.