Open AccessHighly sensitive NADH detection by utilising an aluminium hydroxide/iron hydroxide/MWCNTs nanocomposite film‐modified electrode
Author(s) -
Wang Yu,
Yin Lingling,
Li Xia,
Shang Ran,
Yang Xiangli,
Zhou Xiaoyan,
Chen Yen
Publication year - 2020
Publication title -
micro and nano letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.25
H-Index - 31
ISSN - 1750-0443
DOI - 10.1049/mnl.2020.0073
Subject(s) - detection limit , nanocomposite , hydroxide , biosensor , carbon nanotube , nuclear chemistry , materials science , electrode , inorganic chemistry , chemistry , nanotechnology , chromatography
Glassy carbon electrode (GCE) modified with aluminium hydroxide/iron hydroxide/multi‐walled carbon nanotubes (AH/IH/MWCNTs) composites has been prepared by a simple method and applied for dihydro‐nicotinamide adenine dinucleotide (NADH) detection. AH/IH can not only accelerate electron transfer but also electrostatically interact with the phosphate groups of NADH through iron hydroxide to improve the sensitivity of the sensor. Meanwhile, MWCNTs served as a bonding agent to provide a built‐in conductor, which resulted in boosted electron transfer at the interface. Compared with the GCE, MWCNTs–GCE, and AH/MWCNTs–GCE, the AH/IH/MWCNTs–GCE exhibited an extraordinary electrocatalytic response towards NADH, with a wide linear concentration range from 0.5 to 220 μM with a low‐detection limit of 0.30 μM, at a comparatively low potential (+0.15 V versus Ag/AgCl). Moreover, alcohol dehydrogenase was used as a model system for the design of a sensitive ethanol biosensor. The resulting biosensor exhibited an ethanol sensitivity of 9 μA/mM, a concentration range of 20–400 μM, and a detection limit of 5 μM. These results demonstrate the potential of the AH/IH/MWCNTs nanocomposite film for biosensors in combination with NADH‐producing enzymes.